| Gene | Bdkrb1 |
| Chromosome | 12 |
| Deletion Start | 105,604,180 |
| Deletion End | 105,605,853 |
| Deletion Size | 1,674 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,604,923 | 105,604,944 | ACCCAAGGACAGCAAGACAATG |
| TUP | 105,604,948 | 105,604,975 | CTGATCCTCACACTGGTAGCCTCCTTCC |
| TUR | 105,604,991 | 105,605,014 | CCAGGAAGGCAAAGAAGTGGTAAG |
| TDF | 105,605,280 | 105,605,301 | GCTTCTTGACCAACCGTCTCTG |
| TDP | 105,605,304 | 105,605,329 | ATCATAGGGACCATCTGGTTGGCTGA |
| TDR | 105,605,332 | 105,605,350 | GGCATCTTTGTGGGCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GGTTAGGGATTTAACAATCCG |
| SD | TCATGTGAACTCCAGGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12979A-E4 | VGB6 | C57BL/6NTac | |
| 12979A-B7 | VGB6 | C57BL/6NTac | |
| 12979A-C2 | VGB6 | C57BL/6NTac | |
| 12979A-E2 | VGB6 | C57BL/6NTac | |
