| Gene | Bmi1 |
| Chromosome | 2 |
| Deletion Start | 18,681,815 |
| Deletion End | 18,684,559 |
| Deletion Size | 2,745 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,682,725 | 18,682,748 | GACACTCTTACACTCCACCACAAC |
| TUP | 18,682,750 | 18,682,775 | CCTCATCACTGTGCCTCAACACCAGC |
| TUR | 18,682,779 | 18,682,803 | AGAACTGTTCTAAGGTTTGGGTAGG |
| TDF | 18,683,770 | 18,683,793 | ACCTCATGCATTTTGTGCAGATTT |
| TDP | 18,683,818 | 18,683,836 | TCATTCTGGACCTTAAGAA |
| TDR | 18,683,901 | 18,683,924 | GTCGTCAAGATCTAACCCAAAACG |
PCR Primers
| Primer | Sequence |
| SU | GCTGGAGGACAGCTGAAGAG |
| SD | TGTTATTCACAGTGACGTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 155 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12981A-C3 | VGB6 | C57BL/6NTac | |
| 12981A-D5 | VGB6 | C57BL/6NTac | |
| 12981A-G7 | VGB6 | C57BL/6NTac | |
| 12981A-H11 | VGB6 | C57BL/6NTac | |
