| Gene | Cd14 |
| Chromosome | 18 |
| Deletion Start | 36,726,488 |
| Deletion End | 36,725,293 |
| Deletion Size | 1,196 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,726,442 | 36,726,460 | GGAGCCAACCTGAGGAATC |
| TUP | 36,726,418 | 36,726,440 | CAGGGTCTGGCAGGAGTCTCCCT |
| TUR | 36,726,362 | 36,726,383 | CACCAGAAGCAACAGCAACAAG |
| TDF | 36,725,600 | 36,725,618 | AGGCGCTCCGAGTTGTGAC |
| TDP | 36,725,578 | 36,725,599 | TGGCCCAGTCAGCTAAACTCGC |
| TDR | 36,725,554 | 36,725,576 | TCAGCCCAGTGAAAGACAGATTG |
PCR Primers
| Primer | Sequence |
| SU | ACAGAATGCCCTAATGCCAC |
| SD | ATTTAGCACTGAGCCTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12982A-D1 | VGB6 | C57BL/6NTac | |
| 12982A-D11 | VGB6 | C57BL/6NTac | |
| 12982A-G2 | VGB6 | C57BL/6NTac | |
| 12982A-F9 | VGB6 | C57BL/6NTac | |
