| Gene | Cdx2 |
| Chromosome | 5 |
| Deletion Start | 147,306,979 |
| Deletion End | 147,301,796 |
| Deletion Size | 5,184 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,306,956 | 147,306,977 | CGTGAGCTACCTTCTGGACAAG |
| TUP | 147,306,928 | 147,306,953 | CGTGAGCATGTATCCTAGCTCCGTGC |
| TUR | 147,306,874 | 147,306,893 | ACTGCGGAGGACTGACAAAG |
| TDF | 147,302,698 | 147,302,718 | TCTTGCAGAGGATCGGAGTTC |
| TDP | 147,302,673 | 147,302,697 | TGTTTCCAGCATCCACGTCAGGTGG |
| TDR | 147,302,638 | 147,302,658 | GCAATCAGAGTTAGCCGACAC |
PCR Primers
| Primer | Sequence |
| SU | ATGCAAATCGTGTTTCTGGG |
| SD | GCCAGCTGGTTCTCAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12984A-B4 | VGB6 | C57BL/6NTac | |
| 12984A-G3 | VGB6 | C57BL/6NTac | |
