| Gene | D17H6S53E |
| Chromosome | 17 |
| Deletion Start | 35,127,118 |
| Deletion End | 35,128,005 |
| Deletion Size | 888 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,127,381 | 35,127,398 | CCTCAAGCGGGACAAGAC |
| TUP | 35,127,399 | 35,127,425 | TGCCTCTACCATTCAAGAGCCTGCAAG |
| TUR | 35,127,469 | 35,127,487 | TCACGCCACCTGAATCCAC |
| TDF | 35,127,711 | 35,127,729 | GCCACTCCGAGCCATCTTG |
| TDP | 35,127,732 | 35,127,754 | TGCTTTGGGCCTTCGAGGACCTG |
| TDR | 35,127,782 | 35,127,801 | ATGTAGGGCGGCCAGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | CAATGGAACACCACCTCTCC |
| SD | GTCCGTTCTCCACGGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12987A-A7 | VGB6 | C57BL/6NTac | |
| 12987A-A10 | VGB6 | C57BL/6NTac | |
| 12987A-E8 | VGB6 | C57BL/6NTac | |
| 12987A-D2 | VGB6 | C57BL/6NTac | |
