| Gene | Dhh |
| Chromosome | 15 |
| Deletion Start | 98,898,269 |
| Deletion End | 98,893,904 |
| Deletion Size | 4,366 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,897,956 | 98,897,979 | GATGACAGAGGTAAGGAGAGTTTC |
| TUP | 98,897,930 | 98,897,952 | CCACCTGCTCTACCACACCACCA |
| TUR | 98,897,894 | 98,897,915 | TCATCCTCCAGGGTTGTTGTAG |
| TDF | 98,894,853 | 98,894,872 | CCGGATTCGACTGGGTCTAC |
| TDP | 98,894,830 | 98,894,852 | TACGAGTCCCGCAACCACATCCA |
| TDR | 98,894,788 | 98,894,809 | GACCATCATCTGGCCTCTACTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTGTCCAGTTGTGACTGC |
| SD | TGTTAAAGCCAAGCTCCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12988A-A2 | VGB6 | C57BL/6NTac | |
| 12988A-B8 | VGB6 | C57BL/6NTac | |
| 12988A-D4 | VGB6 | C57BL/6NTac | |
| 12988A-H4 | VGB6 | C57BL/6NTac | |
