| Gene | Dlx1 |
| Chromosome | 2 |
| Deletion Start | 71,529,997 |
| Deletion End | 71,532,612 |
| Deletion Size | 2,616 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,530,068 | 71,530,087 | GCCCAACCAGCAAATGTCTC |
| TUP | 71,530,088 | 71,530,109 | CTTCTCCCATGTCCCACGGGCA |
| TUR | 71,530,124 | 71,530,143 | ATGGCCCGCCGAGTGTAAAC |
| TDF | 71,532,474 | 71,532,492 | CCCTCAGCGCACCAAGAAG |
| TDP | 71,532,495 | 71,532,518 | ATGCAGCAGCCTCAACTGATGTGA |
| TDR | 71,532,542 | 71,532,561 | CAAGGGAGACGGGCAGGAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGCTTCTCGGACCAATCC |
| SD | ATGAGGACAGCAGACACACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12989A-D9 | VGB6 | C57BL/6NTac | |
| 12989A-H10 | VGB6 | C57BL/6NTac | |
| 12989A-F9 | VGB6 | C57BL/6NTac | |
