| Gene | Drd5 |
| Chromosome | 5 |
| Deletion Start | 38,319,669 |
| Deletion End | 38,321,112 |
| Deletion Size | 1,444 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,320,099 | 38,320,117 | GCTACGAGCGCAAGATGAC |
| TUP | 38,320,119 | 38,320,141 | CAGCGTGTAGCCCTGGTCATGGT |
| TUR | 38,320,169 | 38,320,189 | TGGACCGGGATAAAGGAGATG |
| TDF | 38,320,574 | 38,320,597 | GTTGCCTTTCTTCATCCTGAACTG |
| TDP | 38,320,600 | 38,320,622 | TGGTTCCCTTCTGCAGCAGTGGA |
| TDR | 38,320,651 | 38,320,671 | AGGTGGTCTCGCTGACACAAG |
PCR Primers
| Primer | Sequence |
| SU | AGAAGCGTGGCTGATAGGAG |
| SD | TACCAGCAATTGCCACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12991A-H5 | VGB6 | C57BL/6NTac | |
| 12991A-D8 | VGB6 | C57BL/6NTac | |
| 12991A-E8 | VGB6 | C57BL/6NTac | |
| 12991A-G2 | VGB6 | C57BL/6NTac | |
