| Gene | Eif4a1 |
| Chromosome | 11 |
| Deletion Start | 69,672,298 |
| Deletion End | 69,667,216 |
| Deletion Size | 5,083 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,672,222 | 69,672,241 | CCCGTAGTTCCCTCCAAAGG |
| TUP | 69,672,185 | 69,672,207 | AGTTGAGAGGCCCATCGCACCGA |
| TUR | 69,672,163 | 69,672,182 | GTTCTCCCGCAACCTGGTAG |
| TDF | 69,668,191 | 69,668,211 | CACTGTTTCTGCCATGGTGAG |
| TDP | 69,668,156 | 69,668,184 | TGGCTATGTGTCTCTGTGTGTCTGTCTGT |
| TDR | 69,668,108 | 69,668,129 | CGTGCTGGAAAACAAAGAGGTA |
PCR Primers
| Primer | Sequence |
| SU | ACATGACTAAGGCACGATCG |
| SD | TGGCTACCATTTTCTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12992A-E4 | VGB6 | C57BL/6NTac | |
| 12992A-E3 | VGB6 | C57BL/6NTac | |
