| Gene | En2 |
| Chromosome | 5 |
| Deletion Start | 28,166,530 |
| Deletion End | 28,170,505 |
| Deletion Size | 3,976 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,167,161 | 28,167,182 | GCTATTCTGACCGGCCTTCTTC |
| TUP | 28,167,191 | 28,167,211 | CCATGGGAATCAGGCCGCTGA |
| TUR | 28,167,247 | 28,167,266 | TTCCCTCCGTGCAAGTTTAC |
| TDF | 28,170,278 | 28,170,300 | GCCTGAACGAGTCTCAGATCAAG |
| TDP | 28,170,301 | 28,170,325 | ATTTGGTTCCAGAACAAGCGGGCCA |
| TDR | 28,170,333 | 28,170,350 | TTGTTGCCCGTGGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GCGGTGACCATCTAGAGTCC |
| SD | ACAGTCCTGTGAAATGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12993A-B2 | VGB6 | C57BL/6NTac | |
| 12993A-B7 | VGB6 | C57BL/6NTac | |
| 12993A-F1 | VGB6 | C57BL/6NTac | |
| 12993A-D12 | VGB6 | C57BL/6NTac | |
| 12993A-C11 | VGB6 | C57BL/6NTac | |
