| Gene | Evx1 |
| Chromosome | 6 |
| Deletion Start | 52,313,848 |
| Deletion End | 52,317,180 |
| Deletion Size | 3,333 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,314,568 | 52,314,591 | GATTCAGCTAAGACGGAAAGACAC |
| TUP | 52,314,593 | 52,314,615 | TCCGTCGGGTAGGGCAAGATCAA |
| TUR | 52,314,619 | 52,314,636 | TCAAACTGCCCGGACAAC |
| TDF | 52,316,524 | 52,316,544 | TCTCCTCAGGTGTGGTTTCAG |
| TDP | 52,316,547 | 52,316,568 | CCGGCGCATGAAGGACAAGCGT |
| TDR | 52,316,619 | 52,316,638 | CCGCGTGGCTCATCATGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCACTGTGGCAGACGTTTC |
| SD | TCCAAGAAACAGCGAGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12994A-E1 | VGB6 | C57BL/6NTac | |
| 12994A-E8 | VGB6 | C57BL/6NTac | |
| 12994A-F2 | VGB6 | C57BL/6NTac | |
| 12994A-G5 | VGB6 | C57BL/6NTac | |
