Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

12995 Foxs1 Status: ES cell colonies screened / QC positives

Gene	Foxs1
Chromosome	2
Deletion Start	152,933,128
Deletion End	152,932,120
Deletion Size	1,009
Genome Build	38

MGI ID	MGI:95546
NCBI Gene ID	14239
BAC ID	402C20
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	152,932,883	152,932,902	TCAAGGTGCCTCGTGATGAC
TUP	152,932,858	152,932,880	CAAGCCAGGCAAGGGCAGCTACT
TUR	152,932,809	152,932,829	AGAAAGCTGCCATGCTGGAAC
TDF	152,932,395	152,932,412	TGAGAGCGTTGGGAGCAG
TDP	152,932,371	152,932,393	TACCAGTGCCGGATGCAGACACT
TDR	152,932,320	152,932,342	GAAGAGACCAGGAGGTGTTCAAG

PCR Primers

Primer	Sequence
SU	TTTCCTCTGCTGTGTCTCCC
SD	CAACCAGAATCTTTCCAGGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	94 bp	+	+	-
TDF	TDR	93 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
12995A-D1	VGB6	C57BL/6NTac
12995A-E8	VGB6	C57BL/6NTac
12995A-G4	VGB6	C57BL/6NTac
12995A-G11	VGB6	C57BL/6NTac