| Gene | Fut4 |
| Chromosome | 9 |
| Deletion Start | 14,751,993 |
| Deletion End | 14,750,681 |
| Deletion Size | 1,313 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,751,511 | 14,751,530 | CCCGAGAACGCACGGATAAG |
| TUP | 14,751,486 | 14,751,509 | CGCTGGTACTACGCGTGTTCGACG |
| TUR | 14,751,446 | 14,751,465 | TCCAGGGCTTTGCCAGTTAG |
| TDF | 14,750,830 | 14,750,850 | GCTGTCTATCGCCGCTACTTC |
| TDP | 14,750,801 | 14,750,823 | CGCCGGAGCTTTGCAGTGCACAT |
| TDR | 14,750,769 | 14,750,787 | TCCGGCACCATTGCTCATC |
PCR Primers
| Primer | Sequence |
| SU | TAGGCGCAGGAACAACGAAG |
| SD | GAGGAATCCAACCACCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12996A-D3 | VGB6 | C57BL/6NTac | |
| 12996A-D7 | VGB6 | C57BL/6NTac | |
| 12996A-D9 | VGB6 | C57BL/6NTac | |
