| Gene | Hspa5 |
| Chromosome | 2 |
| Deletion Start | 34,772,325 |
| Deletion End | 34,776,155 |
| Deletion Size | 3,831 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,772,670 | 34,772,688 | TGCGGCCAAGAACCAACTC |
| TUP | 34,772,698 | 34,772,721 | CCCGAGAACACGGTCTTCGATGCC |
| TUR | 34,772,728 | 34,772,746 | TCCAAGTGCGTCCGATGAG |
| TDF | 34,775,582 | 34,775,605 | TCTGGGTACATTTGATCTGACTGG |
| TDP | 34,775,608 | 34,775,629 | TTCCTCCTGCTCCCCGTGGAGT |
| TDR | 34,775,658 | 34,775,682 | TCACTCGGAGAATACCATTAACATC |
PCR Primers
| Primer | Sequence |
| SU | ATTGGTGGCCGTTAAGAATG |
| SD | CTACACCACATGTGCATGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12997A-A1 | VGB6 | C57BL/6NTac | |
| 12997A-A9 | VGB6 | C57BL/6NTac | |
| 12997A-B8 | VGB6 | C57BL/6NTac | |
| 12997A-H7 | VGB6 | C57BL/6NTac | |
