| Gene | Hp |
| Chromosome | 8 |
| Deletion Start | 109,579,011 |
| Deletion End | 109,575,189 |
| Deletion Size | 3,823 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,578,622 | 109,578,644 | TCAGAAGAACTTCCTGACTGGTC |
| TUP | 109,578,597 | 109,578,621 | AGCATGTGCAGGCAACTGGGTAGCA |
| TUR | 109,578,566 | 109,578,586 | GGCCATCATTGGTACACAGAC |
| TDF | 109,575,991 | 109,576,011 | CGCATCATCGGTGGCTCTATG |
| TDP | 109,575,962 | 109,575,985 | CAAAGGCAGCTTTCCTTGGCAGGC |
| TDR | 109,575,942 | 109,575,961 | CGTGGCGGGAGATCATCTTG |
PCR Primers
| Primer | Sequence |
| SU | AGCTAAGGCTGGGTCACAAG |
| SD | TTGCATTCAAACTGACCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13004A-A9 | VGB6 | C57BL/6NTac | |
| 13004A-B1 | VGB6 | C57BL/6NTac | |
| 13004A-H10 | VGB6 | C57BL/6NTac | |
| 13004A-D1 | VGB6 | C57BL/6NTac | |
| 13004A-F7 | VGB6 | C57BL/6NTac | |
| 13004A-F5 | VGB6 | C57BL/6NTac | |
