| Gene | Hspa2 |
| Chromosome | 12 |
| Deletion Start | 76,404,537 |
| Deletion End | 76,406,529 |
| Deletion Size | 1,993 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,404,947 | 76,404,965 | CAAGGTGCAGAGCGCAGTC |
| TUP | 76,404,970 | 76,404,997 | CTGTTCCTGCCTATTTCAACGACTCGCA |
| TUR | 76,405,025 | 76,405,044 | ACACGTTGAGGCCGGTGATG |
| TDF | 76,405,811 | 76,405,828 | GCAGACGCAGACCTTCAC |
| TDP | 76,405,830 | 76,405,854 | ACCTACTCAGACAACCAGAGCAGCG |
| TDR | 76,405,863 | 76,405,882 | CGTTCGCCCTCGTACACTTG |
PCR Primers
| Primer | Sequence |
| SU | ACTAGATATCAGTTGGACCAC |
| SD | ACCAAGCTTCCACAGGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13005A-G6 | VGB6 | C57BL/6NTac | |
| 13005A-D8 | VGB6 | C57BL/6NTac | |
| 13005A-F5 | VGB6 | C57BL/6NTac | |
