| Gene | Htr1a |
| Chromosome | 13 |
| Deletion Start | 105,444,257 |
| Deletion End | 105,445,597 |
| Deletion Size | 1,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,444,500 | 105,444,520 | CTCATGGTGTCAGTGCTGGTG |
| TUP | 105,444,522 | 105,444,544 | TGCCCATGGCTGCTCTGTACCAG |
| TUR | 105,444,577 | 105,444,598 | CAGGGCGATAAACAGGTCACAG |
| TDF | 105,445,374 | 105,445,393 | GTTGCCACATGCCTGAGTTG |
| TDP | 105,445,395 | 105,445,420 | TGGGTGCCATAATTAACTGGCTAGGC |
| TDR | 105,445,426 | 105,445,445 | CTGGGTTGAGCAGGGAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GAAGATGCTTTCAGCCCAAC |
| SD | AGCAAGAGGGAGGGAGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13006A-C5 | VGB6 | C57BL/6NTac | |
| 13006A-C11 | VGB6 | C57BL/6NTac | |
| 13006A-H5 | VGB6 | C57BL/6NTac | |
| 13006A-G9 | VGB6 | C57BL/6NTac | |
| 13006A-G7 | VGB6 | C57BL/6NTac | |
| 13006A-B5 | VGB6 | C57BL/6NTac | |
