13008   Ihh     Status: ES cell colonies screened / QC positives

GeneIhh
Chromosome1
Deletion Start74,951,325
Deletion End74,946,084
Deletion Size5,242
Genome Build38
MGI IDMGI:96533
NCBI Gene ID16147
BAC ID308P3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,950,57074,950,588GACCTGGCTGCCTCTTGTG
TUP74,950,54674,950,569CCTCCGGGAACCACGAAATCCCTC
TUR74,950,51974,950,540GCTGGGCTGTGAGAAATGTTAG
TDF74,946,29374,946,314ACTTGTGGTGGAGGATGTGGTG
TDP74,946,27074,946,291CCTCCTGCTTTGCAGCTGTGGC
TDR74,946,20874,946,227CCCATGCCAAACTGGGAAAC

PCR Primers

PrimerSequence
SUAGGCAGGGACATGGATAGG
SDTAGCAGCATCGACTGAGGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR70 bp++-
TDFTDR107 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13008B-A5VGB6C57BL/6NTac
13008B-E9VGB6C57BL/6NTac
13008B-C8VGB6C57BL/6NTac
13008B-B1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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