| Gene | Lalba |
| Chromosome | 15 |
| Deletion Start | 98,482,668 |
| Deletion End | 98,480,640 |
| Deletion Size | 2,029 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,481,973 | 98,481,992 | CCCAGCATGTCTCAGGTGTG |
| TUP | 98,481,942 | 98,481,971 | TGGGTACCATATGACTTGAGATAGCAGTGC |
| TUR | 98,481,918 | 98,481,938 | GACCAGTGACCAGGTTCTTAC |
| TDF | 98,480,776 | 98,480,796 | AAAGTTCCAGCCAGCCCATTC |
| TDP | 98,480,733 | 98,480,760 | TTTGTGATCAGGAAAGCCTACAAGCCCA |
| TDR | 98,480,697 | 98,480,718 | TCTCACAACGCCACTGTTCAAG |
PCR Primers
| Primer | Sequence |
| SU | GGTAAGCATGGAGTTGGCTG |
| SD | GAGTGGCTACGACCTGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13011A-B6 | VGB6 | C57BL/6NTac | |
| 13011A-F7 | VGB6 | C57BL/6NTac | |
| 13011A-E4 | VGB6 | C57BL/6NTac | |
| 13011A-D5 | VGB6 | C57BL/6NTac | |
| 13011A-G9 | VGB6 | C57BL/6NTac | |
| 13011A-B10 | VGB6 | C57BL/6NTac | |
