| Gene | Ascl2 |
| Chromosome | 7 |
| Deletion Start | 142,968,977 |
| Deletion End | 142,967,869 |
| Deletion Size | 1,109 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,968,599 | 142,968,616 | GCCCGTGAAGGTGCAAAC |
| TUP | 142,968,572 | 142,968,594 | ACTTCCCACCGCACCCGGTTCCT |
| TUR | 142,968,530 | 142,968,549 | CCCTGCTACGAGTTCTGGTG |
| TDF | 142,968,246 | 142,968,264 | GCGCCAACAAGAAGCTGAG |
| TDP | 142,968,224 | 142,968,245 | TAAGGTGGAGACGCTGCGCTCC |
| TDR | 142,968,199 | 142,968,217 | GCAGCGCACGAATGTACTC |
PCR Primers
| Primer | Sequence |
| SU | TGGTCACCGCAAAGCTTGG |
| SD | CTAGGACGCGATTTGCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13012A-A3 | VGB6 | C57BL/6NTac | |
| 13012A-H2 | VGB6 | C57BL/6NTac | |
| 13012A-G10 | VGB6 | C57BL/6NTac | |
| 13012A-C4 | VGB6 | C57BL/6NTac | |
| 13012A-C5 | VGB6 | C57BL/6NTac | |
