| Gene | Oxt |
| Chromosome | 2 |
| Deletion Start | 130,576,213 |
| Deletion End | 130,576,946 |
| Deletion Size | 734 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,576,456 | 130,576,477 | CAGGGCTTTGGGAAGTGTTAGG |
| TUP | 130,576,479 | 130,576,501 | TCCCTCTTGACGCCGTGAAGGTA |
| TUR | 130,576,506 | 130,576,524 | AGTGGGTGCTCCGGCATTG |
| TDF | 130,576,847 | 130,576,868 | GCTCAGAACACTGACCCATTTC |
| TDP | 130,576,870 | 130,576,891 | CTTGCAGATGGCTGCCGCACAG |
| TDR | 130,576,909 | 130,576,927 | GCTCCGAGAAGGCAGACTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTCTAGCCTCTTGTAGC |
| SD | ACCGTTGCTGTTTGGACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13019A-C7 | VGB6 | C57BL/6NTac | |
| 13019A-H4 | VGB6 | C57BL/6NTac | |
