| Gene | Pmch |
| Chromosome | 10 |
| Deletion Start | 88,091,140 |
| Deletion End | 88,092,221 |
| Deletion Size | 1,082 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,091,571 | 88,091,590 | GGGAGGTGAACGCAGTGTAG |
| TUP | 88,091,591 | 88,091,615 | AGCTTCTTAGAATTGATCAGCAAGA |
| TUR | 88,091,650 | 88,091,671 | GGTTTGTATCCCAGCTGTCTTC |
| TDF | 88,092,130 | 88,092,153 | TCTCTTTCAGTGCTCAGGTGTATG |
| TDP | 88,092,154 | 88,092,181 | CTGGGAAGAGTCTACCGACCATGTTGGC |
| TDR | 88,092,188 | 88,092,206 | TTGCGGACCAGCAGGTATC |
PCR Primers
| Primer | Sequence |
| SU | GAGCCTGGTATCCTGTGGTC |
| SD | TGTATCCCTGGGAGGACAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13020A-F4 | VGB6 | C57BL/6NTac | |
| 13020B-F5 | VGB6 | C57BL/6NTac | |
| 13020B-G6 | VGB6 | C57BL/6NTac | |
| 13020B-C10 | VGB6 | C57BL/6NTac | |
