| Gene | Pnmt |
| Chromosome | 11 |
| Deletion Start | 98,386,635 |
| Deletion End | 98,388,189 |
| Deletion Size | 1,555 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,386,921 | 98,386,939 | ACCTAGCAGGCACAGACAG |
| TUP | 98,386,941 | 98,386,963 | AGCGGGCCTGTAGTCTTAGCGAC |
| TUR | 98,386,990 | 98,387,008 | TCCAATTCCCGGCCTCAAG |
| TDF | 98,387,771 | 98,387,788 | GCCTTGGTCTCTGCCTTC |
| TDP | 98,387,791 | 98,387,814 | CCTGGAGGCTGTGAGCCCAGATCT |
| TDR | 98,387,837 | 98,387,858 | TCAGCAGTGTGGTGATGTGATG |
PCR Primers
| Primer | Sequence |
| SU | GAGATGCTGGGCAAAGAGTC |
| SD | TGAGGGTGGTAGGGACTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13021A-G2 | VGB6 | C57BL/6NTac | |
| 13021A-G6 | VGB6 | C57BL/6NTac | |
| 13021A-C2 | VGB6 | C57BL/6NTac | |
