| Gene | Ptma |
| Chromosome | 1 |
| Deletion Start | 86,526,909 |
| Deletion End | 86,530,028 |
| Deletion Size | 3,120 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,527,756 | 86,527,775 | CCTCCCGGTGGAGTCTTCTC |
| TUP | 86,527,779 | 86,527,800 | CCTCAGCCGTGTTCGGTTCGGA |
| TUR | 86,527,855 | 86,527,875 | AACAAGGCGACTGGGACTGAG |
| TDF | 86,529,200 | 86,529,221 | GAGGAGGCAGAGAATGGAAGAG |
| TDP | 86,529,222 | 86,529,244 | ATGCACCTGCCAATGGGAACGCT |
| TDR | 86,529,245 | 86,529,265 | CCCAGCTACTTGGACACTCAC |
PCR Primers
| Primer | Sequence |
| SU | TGTTCGGAATCGAGCCAATG |
| SD | CTCTGAAGGCTGGTTTGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13022A-A4 | VGB6 | C57BL/6NTac | |
| 13022A-H8 | VGB6 | C57BL/6NTac | |
| 13022A-E10 | VGB6 | C57BL/6NTac | |
| 13022A-C9 | VGB6 | C57BL/6NTac | |
