| Gene | Rom1 |
| Chromosome | 19 |
| Deletion Start | 8,929,170 |
| Deletion End | 8,927,599 |
| Deletion Size | 1,572 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,928,742 | 8,928,760 | GGCTGCCTTGGCTCACTAC |
| TUP | 8,928,714 | 8,928,737 | ACACAGAGGTGCCTGGACGCTGTC |
| TUR | 8,928,678 | 8,928,700 | ACCTCAACTGCAACTCATCCATC |
| TDF | 8,928,071 | 8,928,089 | GCAGCCCAACCTAAACCTC |
| TDP | 8,928,043 | 8,928,065 | CCAAGGGTGCCATGAAGTGCTGC |
| TDR | 8,928,008 | 8,928,028 | TCCCAGTGTGCCTGATAAACC |
PCR Primers
| Primer | Sequence |
| SU | CGTACCAAACTCACTTCCGC |
| SD | TTTCCTTCATTTGGCAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13023A-A8 | VGB6 | C57BL/6NTac | |
| 13023A-E5 | VGB6 | C57BL/6NTac | |
| 13023A-E7 | VGB6 | C57BL/6NTac | |
| 13023A-G12 | VGB6 | C57BL/6NTac | |
| 13023A-H12 | VGB6 | C57BL/6NTac | |
