| Gene | Snai1 |
| Chromosome | 2 |
| Deletion Start | 167,538,293 |
| Deletion End | 167,542,134 |
| Deletion Size | 3,842 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 167,539,123 | 167,539,142 | CCTTGTGTCTGCACGACCTG |
| TUP | 167,539,151 | 167,539,176 | CCTTCTCTAGGCCCTGGCTGCTTCAG |
| TUR | 167,539,203 | 167,539,223 | AGCTCAGCTATGCACACTCAC |
| TDF | 167,541,603 | 167,541,625 | GAGGTATGGTGGGTGACCTAGAG |
| TDP | 167,541,627 | 167,541,650 | CTGCTGCGTACCTCTGTGCCTCAG |
| TDR | 167,541,704 | 167,541,721 | CTGGCAGGCACTGAACAC |
PCR Primers
| Primer | Sequence |
| SU | CACCTGCTCCGGTCTCAG |
| SD | CCTTGAGGGAGGTAGGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13025A-C8 | VGB6 | C57BL/6NTac | |
| 13025A-D6 | VGB6 | C57BL/6NTac | |
| 13025A-E5 | VGB6 | C57BL/6NTac | |
| 13025A-E3 | VGB6 | C57BL/6NTac | |
| 13025A-E11 | VGB6 | C57BL/6NTac | |
| 13025A-G11 | VGB6 | C57BL/6NTac | |
