| Gene | Tnp1 |
| Chromosome | 1 |
| Deletion Start | 73,015,860 |
| Deletion End | 73,015,249 |
| Deletion Size | 612 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,015,677 | 73,015,701 | GGGAACAGTCAAACACTAGGACTTG |
| TUP | 73,015,651 | 73,015,675 | TCTTCCAGACACCTTCATGGAGCCA |
| TUR | 73,015,623 | 73,015,643 | GGCTGGGATGCTGTTTACTAC |
| TDF | 73,015,337 | 73,015,360 | GGGTGTCCTTGTTGTAAATCTGTC |
| TDP | 73,015,306 | 73,015,332 | CCCACAGCAAGTCGCAATTACCGATCC |
| TDR | 73,015,286 | 73,015,305 | TCATTGCCGCATCACAAGTG |
PCR Primers
| Primer | Sequence |
| SU | AATGACCACGGCTAAAGCAC |
| SD | AAACGGATGAGTGTTCCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13027A-C7 | VGB6 | C57BL/6NTac | |
| 13027A-D9 | VGB6 | C57BL/6NTac | |
| 13027A-H7 | VGB6 | C57BL/6NTac | |
| 13027A-F7 | VGB6 | C57BL/6NTac | |
