| Gene | Tnp2 |
| Chromosome | 16 |
| Deletion Start | 10,788,598 |
| Deletion End | 10,788,074 |
| Deletion Size | 525 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,788,507 | 10,788,531 | ACACCAGTAACCAGTGCAATCAGTG |
| TUP | 10,788,483 | 10,788,502 | TGCAGCCACCACTGCCGGAG |
| TUR | 10,788,382 | 10,788,401 | TTGCAGGTGAGTGTCGAGAG |
| TDF | 10,788,306 | 10,788,324 | GCAAGAGAAAGGCCGTCAG |
| TDP | 10,788,282 | 10,788,304 | AGGCGGAAACGGACTCACAGAGC |
| TDR | 10,788,227 | 10,788,245 | TTGCAGTGTTCCCTTGTCC |
PCR Primers
| Primer | Sequence |
| SU | TTGATGTCACCGTGAGAAGG |
| SD | TCCCTTGTGGATAGGAATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 150 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13028A-A5 | VGB6 | C57BL/6NTac | |
| 13028A-A7 | VGB6 | C57BL/6NTac | |
| 13028A-F6 | VGB6 | C57BL/6NTac | |
| 13028A-H4 | VGB6 | C57BL/6NTac | |
| 13028A-A2 | VGB6 | C57BL/6NTac | |
| 13028A-H12 | VGB6 | C57BL/6NTac | |
