| Gene | Vtn |
| Chromosome | 11 |
| Deletion Start | 78,499,353 |
| Deletion End | 78,502,266 |
| Deletion Size | 2,914 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,499,763 | 78,499,785 | GAGCCCAAGAACAATACCAACAC |
| TUP | 78,499,789 | 78,499,812 | TGTGCAACCCGAGAACACCTCTCC |
| TUR | 78,499,818 | 78,499,838 | CCGTCCGAGGATTTAGGTCAC |
| TDF | 78,502,157 | 78,502,175 | CCTTAGAACCCGGCGAGTG |
| TDP | 78,502,178 | 78,502,202 | CTCTGTGAATCCTCCCTACCCACGC |
| TDR | 78,502,248 | 78,502,266 | GCCGAGTGGGCTCTGATTC |
PCR Primers
| Primer | Sequence |
| SU | CATGCTTTCTTTCCACCTCC |
| SD | ACCCACCTGTTTCTCACGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13032A-B5 | VGB6 | C57BL/6NTac | |
| 13032A-D1 | VGB6 | C57BL/6NTac | |
| 13032A-F9 | VGB6 | C57BL/6NTac | |
| 13032A-H9 | VGB6 | C57BL/6NTac | |
| 13032A-E8 | VGB6 | C57BL/6NTac | |
