| Gene | Cd33 |
| Chromosome | 7 |
| Deletion Start | 43,533,091 |
| Deletion End | 43,528,776 |
| Deletion Size | 4,316 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,533,000 | 43,533,021 | TGGTGGGACAGAACCAAGATAC |
| TUP | 43,532,973 | 43,532,997 | CTCATTTCTTTGCAGGCTCCCTGGC |
| TUR | 43,532,907 | 43,532,926 | TGGACACATAGGCCCTCCTC |
| TDF | 43,529,283 | 43,529,307 | TCTGCCTTCATCTCTTTACCATCTG |
| TDP | 43,529,257 | 43,529,279 | CAGAGCCTCATTGCTACGGCGCT |
| TDR | 43,529,213 | 43,529,232 | CTGAGTCACACCATGCACAC |
PCR Primers
| Primer | Sequence |
| SU | AGTTGAGGAAGGCCTGTGTG |
| SD | ACTTACTCATGGGCAAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 115 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13036A-A4 | VGB6 | C57BL/6NTac | |
| 13036A-A9 | VGB6 | C57BL/6NTac | |
| 13036A-B5 | VGB6 | C57BL/6NTac | |
| 13036A-D9 | VGB6 | C57BL/6NTac | |
