| Gene | Pigh |
| Chromosome | 12 |
| Deletion Start | 79,089,616 |
| Deletion End | 79,082,402 |
| Deletion Size | 7,215 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,089,232 | 79,089,255 | TGGAACATAACCTCCTCAGAGAAC |
| TUP | 79,089,210 | 79,089,231 | TCAGTGCATCCTCCCGCATCAC |
| TUR | 79,089,170 | 79,089,187 | TGTCGCACGCCTGCTATC |
| TDF | 79,082,603 | 79,082,625 | TCCCTCAACCTTGTGTCTCTTAG |
| TDP | 79,082,580 | 79,082,602 | TTCACGGCTGCCATTTAGAGCCA |
| TDR | 79,082,557 | 79,082,579 | CCTTAACCTCTCACATACACCAC |
PCR Primers
| Primer | Sequence |
| SU | GAACAGAGATCTTAATCTGCC |
| SD | TTGTGAAATCTGGTACCAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13038A-A5 | VGB6 | C57BL/6NTac | |
| 13038A-A7 | VGB6 | C57BL/6NTac | |
| 13038A-C10 | VGB6 | C57BL/6NTac | |
| 13038A-G1 | VGB6 | C57BL/6NTac | |
