| Gene | Il1f8 |
| Chromosome | 2 |
| Deletion Start | 24,154,593 |
| Deletion End | 24,159,959 |
| Deletion Size | 5,367 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,155,228 | 24,155,248 | GCCTCACAATCTCCCAGAAAC |
| TUP | 24,155,250 | 24,155,278 | ACAGGGTTCATGACTCACAACAGATGGTA |
| TUR | 24,155,327 | 24,155,347 | ATGCCACCTACCAGGCTTGAC |
| TDF | 24,159,786 | 24,159,805 | AGAGGGCTCCACTTCTGTCT |
| TDP | | | CAGTCAGTCCTCTATC |
| TDR | 24,159,829 | 24,159,851 | ATGGAAGAGGTGGCTATAAACCA |
PCR Primers
| Primer | Sequence |
| SU | CTTAGGGATTGCTGTCCTTG |
| SD | GGGAAACCACATCTCTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13041A-F3 | VGB6 | C57BL/6NTac | |
| 13041B-F3 | VGB6 | C57BL/6NTac | |
