| Gene | Myd88 |
| Chromosome | 9 |
| Deletion Start | 119,339,956 |
| Deletion End | 119,337,324 |
| Deletion Size | 2,633 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,339,204 | 119,339,223 | CCTCTGCTCCGATTGCTCTG |
| TUP | 119,339,180 | 119,339,203 | TGCCCTGCTAGATGCCCAGTTTGG |
| TUR | 119,339,140 | 119,339,159 | TTCCTCCTGCCTTCCCTTTC |
| TDF | 119,337,458 | 119,337,477 | ACAAGGCGATGAAGAAGGAC |
| TDP | 119,337,432 | 119,337,457 | TTTCCCAGTATCCTGCGGTTCATCAC |
| TDR | 119,337,403 | 119,337,424 | TGGTGCAAGGGTTGGTATAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGTAGACTGAGCCTTCGGG |
| SD | TAACAGCCAGTGTGTGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13042A-B3 | VGB6 | C57BL/6NTac | |
| 13042A-B9 | VGB6 | C57BL/6NTac | |
| 13042A-B11 | VGB6 | C57BL/6NTac | |
| 13042A-F5 | VGB6 | C57BL/6NTac | |
