| Gene | Rnf138 |
| Chromosome | 18 |
| Deletion Start | 21,002,063 |
| Deletion End | 21,026,168 |
| Deletion Size | 24,106 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,002,831 | 21,002,851 | TCAGGACAACCAGTGTCAGTG |
| TUP | 21,002,854 | 21,002,879 | AGTTGGAGCCAGCCCTTGAGAACGTA |
| TUR | 21,002,903 | 21,002,920 | GAGGGCTCTGCTCATCAC |
| TDF | 21,025,457 | 21,025,474 | TGCTGCGAAGGATTGGAC |
| TDP | 21,025,475 | 21,025,503 | TCAGGGACTCAGTACATTCTAAACAGCCA |
| TDR | 21,025,551 | 21,025,571 | GCATGGAACACAGCTTTCAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGACTGCGTCTTCTACCTC |
| SD | GCTCTGACAAATGGCTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13043A-A6 | VGB6 | C57BL/6NTac | |
| 13043A-B2 | VGB6 | C57BL/6NTac | |
| 13043A-B3 | VGB6 | C57BL/6NTac | |
