| Gene | Acvr1 |
| Chromosome | 2 |
| Deletion Start | 58,479,920 |
| Deletion End | 58,447,512 |
| Deletion Size | 32,409 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,479,571 | 58,479,589 | TGCGTAGCCAGAAGCATTC |
| TUP | 58,479,549 | 58,479,570 | TTCCGACTTGCTGAGCCAGAGC |
| TUR | 58,479,499 | 58,479,520 | AGGCCATGCCTTTGTCTTCCTC |
| TDF | 58,447,663 | 58,447,685 | TCCTCTCCACAGACATTAACTTC |
| TDP | 58,447,637 | 58,447,662 | TCTGGCGAAGCTGATGAAAGAGTGCT |
| TDR | 58,447,614 | 58,447,635 | TCTTGCGGATGGGTTCTGATAC |
PCR Primers
| Primer | Sequence |
| SU | TCTGTGCTAGCATAATCCTC |
| SD | CGAAAGATACGCAGAGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13048A-E2 | VGB6 | C57BL/6NTac | |
| 13048A-A3 | VGB6 | C57BL/6NTac | |
