| Gene | Rtn4 |
| Chromosome | 11 |
| Deletion Start | 29,693,192 |
| Deletion End | 29,733,833 |
| Deletion Size | 40,642 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,693,860 | 29,693,880 | TGGGTGCCTTCATTGTTTGTC |
| TUP | 29,693,895 | 29,693,916 | CACGTGCACCGCTGCCAAGTCG |
| TUR | 29,693,939 | 29,693,957 | GCTGCTGATAGGCGAGAAC |
| TDF | 29,733,676 | 29,733,697 | GGTGTTTGGTGCCAGCTTATTC |
| TDP | 29,733,699 | 29,733,724 | TGCTGCTGTCTCTGACAGTGTTCAGC |
| TDR | 29,733,734 | 29,733,755 | CCAAGGCAATGTAGGCCGTTAC |
PCR Primers
| Primer | Sequence |
| SU | CATCTTCTCTATCCCGCAGC |
| SD | AGAAACTGAAGCAAATGTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13050A-D3 | VGB6 | C57BL/6NTac | |
| 13050A-E10 | VGB6 | C57BL/6NTac | |
| 13050A-B10 | VGB6 | C57BL/6NTac | |
