| Gene | Col10a1 |
| Chromosome | 10 |
| Deletion Start | 34,390,625 |
| Deletion End | 34,396,112 |
| Deletion Size | 5,488 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,390,659 | 34,390,678 | TGACCCTGGTTCATGGGATG |
| TUP | 34,390,684 | 34,390,706 | TGCTGAACGGTACCAAACGCCCA |
| TUR | 34,390,740 | 34,390,762 | GGCGTATGGGATGAAGTATTGTG |
| TDF | 34,396,021 | 34,396,040 | ACTCCTCTGAGTACGTCCAC |
| TDP | 34,396,041 | 34,396,066 | TCGTCCTTCTCAGGATTCCTAGTGGC |
| TDR | 34,396,086 | 34,396,108 | GCGGAGAATAGGATGAGCTTGAC |
PCR Primers
| Primer | Sequence |
| SU | AAGTCATGTCAAAGACTCTGG |
| SD | TTCCTGCATGTTTCCTAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13051A-C2 | VGB6 | C57BL/6NTac | |
| 13051A-C10 | VGB6 | C57BL/6NTac | |
| 13051A-E3 | VGB6 | C57BL/6NTac | |
| 13051A-H9 | VGB6 | C57BL/6NTac | |
