| Gene | Acvr2a |
| Chromosome | 2 |
| Deletion Start | 48,870,306 |
| Deletion End | 48,873,471 |
| Deletion Size | 3,166 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,870,335 | 48,870,359 | TGCTAATTGGGAAAGAGACAGAACC |
| TUP | 48,870,362 | 48,870,389 | CCAGACTGGTGTTGAACCTTGCTATGGT |
| TUR | 48,870,455 | 48,870,475 | TCCAGCCAACAACCTTGCTTC |
| TDF | 48,873,241 | 48,873,265 | CCTGGCTCTCATCTATGGTTACTAC |
| TDP | 48,873,270 | 48,873,296 | AGGTTTGTGTAGACCAAATCCGAGCCA |
| TDR | 48,873,331 | 48,873,350 | GGGTGGCTTCGGTGTAACAG |
PCR Primers
| Primer | Sequence |
| SU | TAGGCAATTTAGATTACTGTGG |
| SD | CATACAAAATTAGTAAGACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 141 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13054A-B9 | VGB6 | C57BL/6NTac | |
| 13054A-E10 | VGB6 | C57BL/6NTac | |
