13057   Tex2     Status: ES cell colonies screened / QC positives

GeneTex2
Chromosome11
Deletion Start106,568,599
Deletion End106,544,181
Deletion Size24,419
Genome Build38
MGI IDMGI:102465
NCBI Gene ID21763
BAC ID222F3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF106,568,097106,568,119CCACTGTCTTCTCCTTCCAAGTC
TUP106,568,071106,568,093TGTCCTGTCCTCCAGTGCCTCGT
TUR106,568,002106,568,021TTGGCTCCACCTCAGTTGAC
TDF106,544,775106,544,798GCTTCATGTGAGATGGCATCTTTG
TDP106,544,748106,544,771TGCCCTGAGCTGTCCATGAACAGT
TDR106,544,690106,544,710GTCAGGGTGGAGCTGTGAATG

PCR Primers

PrimerSequence
SUTGCAAGTGAGACAGATTGTTG
SDTGTGGGACCTTTGTAGACAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR118 bp++-
TDFTDR109 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13057A-D10VGB6C57BL/6NTac
13057A-F5VGB6C57BL/6NTac
13057A-G4VGB6C57BL/6NTac
13057A-C1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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