| Gene | Mtnr1a |
| Chromosome | 8 |
| Deletion Start | 45,069,213 |
| Deletion End | 45,088,110 |
| Deletion Size | 18,898 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,069,327 | 45,069,345 | ACCATCGTGGTGGACATTC |
| TUP | 45,069,346 | 45,069,367 | TGGGCAACCTGCTGGTCATCCT |
| TUR | 45,069,380 | 45,069,403 | CCTGAGTTCCTGAGCTTCTTGTTG |
| TDF | 45,087,458 | 45,087,480 | TCCTGATATGGATGCTGACACTC |
| TDP | 45,087,483 | 45,087,505 | CGCCATCATGCCCAACCTGCAAA |
| TDR | 45,087,546 | 45,087,566 | GAGCTGACAGACTGGGTGAAG |
PCR Primers
| Primer | Sequence |
| SU | TTCCGGATAAGACATCCAGG |
| SD | CCATGGTCCCTCTTGTCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13060A-B7 | VGB6 | C57BL/6NTac | |
| 13060A-F11 | VGB6 | C57BL/6NTac | |
| 13060A-C9 | VGB6 | C57BL/6NTac | |
| 13060A-C10 | VGB6 | C57BL/6NTac | |
| 13060A-F5 | VGB6 | C57BL/6NTac | |
| 13060A-E1 | VGB6 | C57BL/6NTac | |
