| Gene | Prg2 |
| Chromosome | 2 |
| Deletion Start | 84,981,341 |
| Deletion End | 84,983,568 |
| Deletion Size | 2,228 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,982,261 | 84,982,278 | GCAAGACCTGTCGCTACC |
| TUP | 84,982,280 | 84,982,306 | CCTAGTCAGGAGGGCTGAATGCTTTGA |
| TUR | 84,982,321 | 84,982,339 | AGCCTCAGCCACAGACCAC |
| TDF | 84,983,002 | 84,983,023 | CAGAGAATGGGTTGGTGATGAC |
| TDP | 84,983,024 | 84,983,051 | CACTGTGGACCATTAGTTCATCTGCCCA |
| TDR | 84,983,052 | 84,983,073 | ACCACAGGACCATGATGGATAC |
PCR Primers
| Primer | Sequence |
| SU | AGCTGCTCCTTATGGTCTGC |
| SD | ATGTTTGGAAGCTGTGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13062A-C9 | VGB6 | C57BL/6NTac | |
| 13062A-F1 | VGB6 | C57BL/6NTac | |
| 13062A-H7 | VGB6 | C57BL/6NTac | |
| 13062A-F12 | VGB6 | C57BL/6NTac | |
