| Gene | Pdia4 |
| Chromosome | 6 |
| Deletion Start | 47,813,301 |
| Deletion End | 47,796,480 |
| Deletion Size | 16,822 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,813,136 | 47,813,154 | CAGGGTGCTTCGCTCACAC |
| TUP | 47,813,112 | 47,813,135 | CCCTGCGGAATTAGTGACCCGGTA |
| TUR | 47,813,077 | 47,813,096 | TCGGCCCTACACTGAAACTC |
| TDF | 47,796,698 | 47,796,721 | GGCAAGAAATACAAGGGCCAGAAG |
| TDP | 47,796,672 | 47,796,696 | ACCTGGTCATCGCCAAGATGGATGC |
| TDR | 47,796,646 | 47,796,666 | GGTCGTTGGTGATGTCGTTGG |
PCR Primers
| Primer | Sequence |
| SU | CTTACACGTGTTCTTCCTGCC |
| SD | AACAACATGATTTGGCAAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13067A-B11 | VGB6 | C57BL/6NTac | |
| 13067A-C3 | VGB6 | C57BL/6NTac | |
| 13067A-E9 | VGB6 | C57BL/6NTac | |
| 13067A-E11 | VGB6 | C57BL/6NTac | |
