| Gene | Ltbr |
| Chromosome | 6 |
| Deletion Start | 125,313,682 |
| Deletion End | 125,307,219 |
| Deletion Size | 6,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 125,313,208 | 125,313,229 | GGGACAAAGAGACCAAGGAGAG |
| TUP | 125,313,183 | 125,313,206 | CTCCGAGGCATGCAGCAAGCAGAG |
| TUR | 125,313,135 | 125,313,159 | GGAAAGAGGGTTATCAGGAGACAAG |
| TDF | 125,307,303 | 125,307,321 | CTGGCCCTTCTGAGCTGTC |
| TDP | 125,307,280 | 125,307,301 | ACACCCTACCAGGAGGATGGCA |
| TDR | 125,307,233 | 125,307,254 | GAGGTCTTGGCATCCTAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | CTTCCGAAGAAAGGTGGAGG |
| SD | CCCTCCTCTGCCATGTTTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13068A-B8 | VGB6 | C57BL/6NTac | |
| 13068A-C2 | VGB6 | C57BL/6NTac | |
| 13068A-G11 | VGB6 | C57BL/6NTac | |
| 13068A-E3 | VGB6 | C57BL/6NTac | |
| 13068A-F9 | VGB6 | C57BL/6NTac | |
| 13068A-F10 | VGB6 | C57BL/6NTac | |
