| Gene | Lepr |
| Chromosome | 4 |
| Deletion Start | 101,728,033 |
| Deletion End | 101,752,199 |
| Deletion Size | 24,167 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,728,139 | 101,728,163 | ATGTTGACTGATACCTGGTACTTTG |
| TUP | | | TGGTCCCAACCCAT |
| TUR | 101,728,183 | 101,728,207 | TATGGAACTACAGAACAGCTATTCG |
| TDF | 101,751,606 | 101,751,630 | GGTGTTTCCTCCTTTAGTTTGTCAG |
| TDP | 101,751,631 | 101,751,654 | CGATGCATTGACTCATGCCCTGCA |
| TDR | 101,751,674 | 101,751,696 | TGCACAGAAGAGCTATACCAATG |
PCR Primers
| Primer | Sequence |
| SU | TTCACCATAATACTACCTCACGC |
| SD | AACAGATTAGCCCGCTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13069A-D3 | VGB6 | C57BL/6NTac | |
| 13069A-G2 | VGB6 | C57BL/6NTac | |
| 13069A-E11 | VGB6 | C57BL/6NTac | |
