| Gene | Fut7 |
| Chromosome | 2 |
| Deletion Start | 25,424,769 |
| Deletion End | 25,425,942 |
| Deletion Size | 1,174 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,425,095 | 25,425,115 | AGCTGCCGTCTGAGTGCTAAC |
| TUP | 25,425,116 | 25,425,136 | CGGAGCCTGCTAGCCAGTGCT |
| TUR | 25,425,137 | 25,425,156 | TGGTGGAAGACCACAGCATC |
| TDF | 25,425,512 | 25,425,529 | TGTCTGCTGCCCACTTTG |
| TDP | 25,425,536 | 25,425,560 | TACCGCTTCTACCTGGCCTTTGAGA |
| TDR | 25,425,585 | 25,425,605 | CATTGCGCCAGAACTTCTCAG |
PCR Primers
| Primer | Sequence |
| SU | TTGTCTTGGCCTTCCTCTTG |
| SD | CCTTGAGGCTAAGCACCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13075A-H9 | VGB6 | C57BL/6NTac | |
| 13075A-E3 | VGB6 | C57BL/6NTac | |
| 13075A-H3 | VGB6 | C57BL/6NTac | |
| 13075A-C11 | VGB6 | C57BL/6NTac | |
