| Gene | Fam3c |
| Chromosome | 6 |
| Deletion Start | 22,343,275 |
| Deletion End | 22,308,349 |
| Deletion Size | 34,927 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,342,476 | 22,342,500 | AAAGGGTAGACCCCTAAATTCTCAG |
| TUP | | | ACCTTTCCCTACCTACA |
| TUR | 22,342,424 | 22,342,444 | TGGAGGCAGCATAGGAGAAAC |
| TDF | 22,308,433 | 22,308,452 | CTGACGGGAAGGAGCGAGAG |
| TDP | 22,308,406 | 22,308,431 | CGAGGAGGAACGCACTTTGACTCTGC |
| TDR | 22,308,350 | 22,308,368 | GGACGGCCACATGCTACTG |
PCR Primers
| Primer | Sequence |
| SU | TGTATCTTGTACCGGCATCG |
| SD | GGGTACTGCCATAACATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13076A-B10 | VGB6 | C57BL/6NTac | |
| 13076A-B2 | VGB6 | C57BL/6NTac | |
| 13076A-A7 | VGB6 | C57BL/6NTac | |
