| Gene | Slc8a3 |
| Chromosome | 12 |
| Deletion Start | 81,316,040 |
| Deletion End | 81,314,265 |
| Deletion Size | 1,776 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,315,438 | 81,315,456 | GATCAAGCACCTGCGAGTC |
| TUP | 81,315,416 | 81,315,437 | TTCTTCGTCACGGCTGCTTGGA |
| TUR | 81,315,392 | 81,315,414 | AGAGCCAAATGTAGGCGAAGATG |
| TDF | 81,314,907 | 81,314,924 | GCCGAGCAAGCCAAGAAG |
| TDP | 81,314,882 | 81,314,905 | CCTCCAGCATGAGCGAGGTGCATA |
| TDR | 81,314,823 | 81,314,847 | GCACTGATAAGAACATGGGTCAAAG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCATCCCTAGTCTTGCC |
| SD | TAGAACCTTTTGACACCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13078A-B4 | VGB6 | C57BL/6NTac | |
| 13078A-C4 | VGB6 | C57BL/6NTac | |
| 13078A-A6 | VGB6 | C57BL/6NTac | |
