| Gene | Ei24 |
| Chromosome | 9 |
| Deletion Start | 36,793,332 |
| Deletion End | 36,780,024 |
| Deletion Size | 13,309 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,792,823 | 36,792,842 | TTGCGTGGTATGTGCTTGTG |
| TUP | 36,792,792 | 36,792,819 | ATGCAGATGCAGTCCTATTCTCATGGGA |
| TUR | 36,792,730 | 36,792,750 | GGCGAGAAAGCAGACAGAAAC |
| TDF | 36,780,128 | 36,780,148 | CATCCTTCTCCTGCCAAACTG |
| TDP | 36,780,105 | 36,780,127 | AAAGCTGCTGCAGGCCACTGAGC |
| TDR | 36,780,056 | 36,780,075 | AGAGCTGCCACATCCTCCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCAGCAAGTGTGGGACTAG |
| SD | GAGGGAGTGACTGGAGCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13079A-E11 | VGB6 | C57BL/6NTac | |
