| Gene | Vnn1 |
| Chromosome | 10 |
| Deletion Start | 23,894,879 |
| Deletion End | 23,904,720 |
| Deletion Size | 9,842 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,895,529 | 23,895,549 | CCCACAGAGGTCAGACGAAAG |
| TUP | 23,895,553 | 23,895,577 | TGGATCTCCCGGAACTGTTTCAGGA |
| TUR | 23,895,579 | 23,895,596 | CACAGAGCAGCTCCCAAC |
| TDF | 23,904,538 | 23,904,559 | GGGCTCTTTGGGAGGTTGTATG |
| TDP | 23,904,563 | 23,904,588 | AGGACTGGGCATCCAATGCTTCCTCA |
| TDR | 23,904,589 | 23,904,609 | CAGCGAGTGTGCTATGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | GGCTGAAACTGGCAGATAGC |
| SD | TCTGGTCCATGAGAGAACGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13080A-A7 | VGB6 | C57BL/6NTac | |
| 13080A-C5 | VGB6 | C57BL/6NTac | |
| 13080A-D12 | VGB6 | C57BL/6NTac | |
| 13080A-F8 | VGB6 | C57BL/6NTac | |
