| Gene | Oxtr |
| Chromosome | 6 |
| Deletion Start | 112,489,794 |
| Deletion End | 112,476,981 |
| Deletion Size | 12,814 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,489,480 | 112,489,501 | GGACATCACCTTCCGCTTCTAC |
| TUP | 112,489,454 | 112,489,476 | CCCGACCTGCTGTGTCGTCTGGT |
| TUR | 112,489,425 | 112,489,443 | CGAACATGCCCACCACCTG |
| TDF | 112,477,864 | 112,477,881 | ACCTCTCACTGGGCACTC |
| TDP | 112,477,839 | 112,477,862 | TGTGCACCTGACACTCTTTGGTCC |
| TDR | 112,477,775 | 112,477,799 | CACTTCAGTCCCTTCATCTTTACAC |
PCR Primers
| Primer | Sequence |
| SU | TCACCACCGAGAAGTCACC |
| SD | TTAGCACCACTCTGCGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13082A-C7 | VGB6 | C57BL/6NTac | |
| 13082A-F4 | VGB6 | C57BL/6NTac | |
| 13082A-G4 | VGB6 | C57BL/6NTac | |
| 13082A-F6 | VGB6 | C57BL/6NTac | |
| 13082A-G5 | VGB6 | C57BL/6NTac | |
| 13082A-F11 | VGB6 | C57BL/6NTac | |
